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How to calculate LOD & LOQ in HPLC Validation Method

Answer Posted / abdulkalim

Based on standard deviation of the response and the slope
obtained will be calculated as:

Limit of Detection (LOD):
DL= 3(SD/Slope)


Limit of quantification(LOQ):
DL=10(SD/Slope)

Is This Answer Correct ?    231 Yes 20 No



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