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Aristotle
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A blastomere was removed from an 8-cell human embryo, and its beta-globin gene was amplified by PCR. The beta-globin DNA was treated with an enzyme that cuts on either side of and within the normal allele (producing a small fragment), but not within the sickle cell allele (producing a large fragment). Electrophoresis of the treated DNA from the blastomere demonstrated the presence of both a large and small fragment derived from the beta-globin genes. How should you interpret this test?
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The ABO blood system has been employed to settle cases of disputed paternity. Suppose, as an expert in genetics, you are called to testify in a case where the mother is type A, the child is type O and the alleged father is type B. How would you respond to the following statements of the attornies: a. "Since the mother has type A, the type O of the child must have come from the father and since my client is type B, he obviously could not have fatered the child." Made by the attorney of the alleged father. b. "Further tests revealed that this man is heterozygous and therefore he must be the father." Made by the mother's attorney.
