southern blot technique is related to---------
[fill the gaps]
Answer Posted / priya
DNA
it is a technique which involves the transfer of DNA from
gel to nitrocellulose membrane.
Is This Answer Correct ? | 1 Yes | 0 No |
Post New Answer View All Answers
What is the completing sequence of nitrogen-containing bases for an agccgttaac fragment of a dna chain?
Which element has maximum catenation ability?
What is the importance of the primary structure?
What does thermodynamics helps in predicting?
Which enzymes in cows allow them to digest cellulose?
What is the structural formula of molybdenum blue?
Which are the most abundant metal and third abundant element in the earth crust?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
In double bond, how many sigma and Pi bonds are present in it?
What are the chemical bonds of the dna molecule that are broken for the replication process to occur?
Give examples of tautomers.
Concerning their biological function what is the difference between dna and rna?
What is the enzyme that catalyzes the process for production of rna?
What is the difference between essential and natural amino acids?
In which forms arsenic impurities present in drug.