Suppose that an investigator wanted to use DNA
fingerprinting to associate a blood sample with a criminal.
What materials would be necessary?
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Answer Posted / swapna
In RFLP analysis, the DNA of an organism is cut up into
fragments using restriction enzymes. A large number of
short fragments of DNA will be produced.
Restriction enzymes always cut at the same base sequence.
Because no two individuals have identical DNA, no two
individuals will have the same length fragments. For
example, the enzyme EcoRI always cuts DNA at the sequence
GAATTC.
Different people have different numbers of this particular
sequence and will therefore have different fragment
lengths. some of them will be at different locations on
the chromosome.
DNA were placed on the gelatin.
The DNA bands must be stained to make them visible.
Ethidium bromide-stained DNA will fluoresce when
illuminated with UV light.
PCR techniques are used to produce sufficient quantities of
DNA for this technique.
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