A blastomere was removed from an 8-cell human embryo, and
its beta-globin gene was amplified by PCR. The beta-globin
DNA was treated with an enzyme that cuts on either side of
and within the normal allele (producing a small fragment),
but not within the sickle cell allele (producing a large
fragment). Electrophoresis of the treated DNA from the
blastomere demonstrated the presence of both a large and
small fragment derived from the beta-globin genes. How
should you interpret this test?
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The village of Oblong has 400 normally-shaped residents. Transferrin is an iron carrier found in blood, and electrophoretic variation of this protein is determined by an autosomal codominant system. The citizens of Oblong were typed for their transferrin complements. The distribution of transferrin phenotypes was: 100 CC, 100 CD, and 200 DD. What is the frequency of the C allele?
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