How do you customise database for blast?
- 1 Answers
- 3533 Views
- I also Faced
- E-Mail Answers
Step 1. Assemble your sequences.
Put all your sequences in Fasta format in a single file.
This file should be located in a suitably named
subdirectory of your home directory on the UBiC Blast
server. The definition line for each sequence should start
with a unique identifier for that sequence.
Step 2. Convert this sequence file to a Blastable database.
The command formatdb converts your Fasta file of sequences
to a set of files that can be queried with command-line
BLAST.
Step 3. Test Blast on your database.
See the UBiC tutorial, Using Command-line BLAST. In the
blastall command-line you will need to specify the location
of your database by typing: -
d /disk2/home/myhome/blastdbs/custom.aa.
| Is This Answer Correct ? | 1 Yes | 0 No |
If a match from a sequence database search is reported to have an E-value of 0.0, should it be considered highly insignificant or highly significant?
How to Count number of nucleotide substitutions ?
what is the principle involved in Biometry?
Does multidrug resistance (mdr) arise by activation of stable genes encoding drug efflux pumps or by mutations of genes encoding other types of transporters in bacterial pathogens?
Integrate 3x + 5 / (x3-x2-x+1)?
Which of the following is a recursive set of production (a) S ?> a|A, A ?> S (b) S ?> a|A, A ?> b (c) S ?>aA, A?>S (d) None of these.
What is Medical Informatics?
What are the main signals used for gene finding in prokaryotic genomes? How are these signals introduced into the search algorithms?
what is Genome annotation?
A constant voltage is applied between the 2 ends of a uniform metallic wire. Some heat is developed in it. The heat developed is doubled if (a) both the length and radius of the wire are halved. (b) both the length and radius of the wire are doubled (c) the radius of the wire is doubled (d) the length of the wire is doubled.
what is a Homologue?
Describe the aim of microarray data analysis?
Micro Biology 
