if there is solution , how will u estimate that it contains
DNA only, not RNA? how we differentiate DNA ,RNA and
Protein in solution without studying its structure?
- 7 Answers
- 9950 Views
- IIT, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / tk
Spectrophotometrically.OD at 260nm-DNA,280 for proteins
| Is This Answer Correct ? | 44 Yes | 5 No |
Answer / jyothi
alkaline hydrolysis -rna is hydrolysed and become less
viscous while dna has no change .. when we shake a tube
containing pure proteins,it frothes... also takin OD at
260nm is a good option for distinguishing between dna and
protein .
pliz mail me if there is any mistake in this answer. thanx.
| Is This Answer Correct ? | 21 Yes | 3 No |
Answer / abhinav trivedi
just pour the solution in CsCl coloumn, and you will get
different bands. Also there is one very easy method to
detect protein solution from other two by just shaking. on
shaking solution containing protein will start foam
formation while those of DNA n RNA containing will not.
| Is This Answer Correct ? | 4 Yes | 1 No |
Answer / anuj
staining methods for DNA and RNA run on gel for DNA and RNA
for protein acylamide gel
| Is This Answer Correct ? | 0 Yes | 5 No |
Answer / aravind
value of od at240 / od at 260 = 0.8 for all nucleic acid.
| Is This Answer Correct ? | 0 Yes | 5 No |
Answer / shilpa m
Take 3 testtubes and pour DNA,RNA and protines. Subjecte
these solutions to colory meter or spectro photo meter and
the obsrebence of these solutions will be different because
of DNA is double standard and it obserbs more light and it
give od 280 nano meters. coming to RNA, it gives more od
compare to DNA because of its a single standard. coming to
protines gives more od, because of it contains free amino
acids.
Finally these 3 solutions depends on the od.
another way to find density gradiant centrifigation of
these three molecules separated by their molecular weights.
| Is This Answer Correct ? | 2 Yes | 8 No |
what is biotechnology?
List out the biochemical tests with the significance which will be used to identify and characterize a unknown bacteria?
why pupil of the eye black
What is a "transgenic" organism?
Hi guys.My friend has visa appointment in this month but the problem is she applied for the university with her old toefl score and she wrote toefl after she applied and her toefl date expired last month but she did'nt received her official score report till now.Is there any problem if she attended the visa interview with his old score
what type of questions are asked in interview for biotechnology student?
why methionin act as first aminoacid in protein synthesis?
What is the U.S. Government response to the recent findings related pollen from transgenic plants affecting the larvae of Monarch butterflies?
What is isotopic tracer technique?
4 Answers ABC, KOL, Ranbaxy, Vimta Labs,
How is this bio technology useful in our day to day life?
what type of questions asked in interviews
what are your strenths & weaknesses
Micro Biology 
