Answer / sai charitha

proteins are separated considering their molecular
weights.different ph gradients are used,when they are
passed applying electricity,it accumulates at a point at
which it corresponds to its ph value.ampholytes are used
for making gel

Name the bond that resists denaturation?

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What is the relation between reaction taking place in one of the several stages and the total amount of energy liberated in the reaction?

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An unusual structure is formed at the 5? end of each cellular mRNA. What is it? After this has formed, does the mRNA have a 5? terminus? Explain.

1 Answers   Pace Pharmaceuticals,

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WHAT IS POLENSKE NUMBER?

1 Answers   Biological.e.limited,

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What is the role of PAPS?

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What was the primary antibody that was used?

1 Answers   Pace Pharmaceuticals,

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I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

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what is meant by palindromic dna?

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What is allotropy?

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What are the factors affection Respiration ?

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How can one use the pH and the pKa to determine the acid-base form of a compound?

1 Answers  

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What is the tertiary structure of a protein? What are the main types of tertiary structure?

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