what is the concept behind central dogma of molecular genetics?

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what is rf?how do u measure rf?

2 Answers   Reddy Labs,

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What are enzymes? What is the importance of enzymes for the living beings?

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What are nucleic acids? What is the historic origin of this name?

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What are some commonly used biochemical buffers?

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What is the function of csf?

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I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

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Which is the most reactive element in nitrogen family?

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Who were james watson, francis crik and maurice wilkins?

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what are the symptoms of Adrenal Virilism ?

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Why is it usually necessary to cleave proteins into smaller fragments for sequencing?

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Which compound is involved in reducing levels of homocysteine in the blood?

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