What are the main theoretical models that try to explain the formation of the enzyme-substrate complex?
- 0 Answers
- 572 Views
- I also Faced
- E-Mail Answers
No Answer is Posted For this Question
Be the First to Post Answer
what is non competetive inhibition?
How the pure culture can be produced?
what happens when aminoacid reacts with edman reagent?
what are the two types of intermedins ?
Describe the classification of all amino acids .
what are the steps involved in the formation of osazone from glucose?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
you have a mixture of dNTPs(deoxynucleotide triphosphates)in a sample.how can you separate these all?
Define threshold energy?
What is betapropiolactone?
What is le bel-van?t Hoff rule?
what are the different types of monoterpenes?
Micro Biology 
