What is saytzeff’s rule?
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What is the molecular formula of phosphorous?
difference between angiotensin-1 and angiotensin-2?
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I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
What are isozymes?
which is more important, TCA or glycolysis??
What is xanthoproteic test?
Because of dna replication, two dna molecules come to existence. Why is not it correct to assert that two “new” dna molecules are created? What is the name given to the process concerning that fact?
What are allosteric enzymes?
A principal difference between prokaryotic and eukaryotic DNA replication is a) completely different proteins/enzymes in eukaryotes b) multiple origins in eukaryotes c) no requirement for topoisomerase activity in prokaryotes d) the absence of a nucleus in prokaryotes
What is the name of the dna duplication process?
What is the pore size of nitro cellulose memberane used in soythern blotting technique?
What is the enzyme that catalyzes the process for production of rna?