What is the primary structure of a protein?
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what is the difference between hydrolase, synthase and synthetase
What are respectively some remarkable functions of albumin?
How does a buffer work?
What is the name for magnesium per chlorate and what is its formula?
What are similarities and differences between the transcription process and the replication processes?
What is meant by “cooperative folding” of proteins?
what is the primary purpose of fermentation in the absence of oxygen?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
How can one use the pH and the pKa to determine the acid-base form of a compound?
Who proposed law of mass action? What does it states?
which is more important, TCA or glycolysis??
what are the two types of intermedins ?