Interested to Buy Any Domain ? << Click Here >> for more details...
what is the difference of 293T, HEK293 and HEK293T?
- 1 Answers
- 16829 Views
- Anthem, I also Faced
- E-Mail Answers
Answer / andrew
They are all Human Embryonic Kidney cells (well they are supposed to be, but there is some dispute over their actual cell type with some suggestions they may be neuronal). HEK293 are the original cell line derived and are used for the easy transfectability. HEK293T cells have been transduced with the SV40 (virus) large T antigen, which permits the amplification of plasmids containing the SV40 origin of replication. Thus even if only one plasmid makes it into these cells, many copies can be made permitting both higher levels of expression and longer retention of the plasmid following multiple rounds of mitosis.
| Is This Answer Correct ? | 11 Yes | 0 No |
Give the cell cycle analysis of transiently transfected cells ?
What is the formula of the DPD for withered (shrank) plant cells? How is that situation possible?
what is the best cell culture media for in vivo imaging ?
Describe the Glycyrrhizin (GL) and cell culture ?
what happens culture media left out for too longer periods?
What is a membrane?
How to avoid contamination of primary cells during preparation?
What are the chemical substances that compose the plasma membrane?
What is the difference between the passage number of a cell line and its population doubling level?
Where in the cell can ribosomes be found? What is the main biological function of ribosomes?
How many cells (nuclei) per ml pellet?
What are plant cell vacuoles? What are their functions?
Micro Biology 
