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How to know that bacteria have a plasmid?
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Answer / bunty, ku (2006-2008)
1) Expose the bacteria to an antibiotic that kills cells
which dont have the plasmid
2) Use an electron microscope
3) Take out the DNA from al lthe cells to see which ones
have the plasmids
4) Make antibodies for each bacterium that has a recombinent
plasmid.
| Is This Answer Correct ? | 3 Yes | 0 No |
Answer / korokoro
My advisor suggest,
1. TE buffer 20 ul(in TE has Tris that preserve DNA) +
Phenol 5 ul + 1 colony
2. vortex mix about 2-3 min (or until cell lysate,
afterthat centrifuge at 12000 rpm 5 min or just spindown
3. run gel eletrophoresis compare with positive control and
negative control
Principle is lysated cell to check DNA band compare with
same strain bacteria that contain target plasmid and this
method i used to screen transformant()
(but... it's not work for me because positive and negative
control has same band)
Anyone have a rapid and simple method to screen plasmid in
transformant?
| Is This Answer Correct ? | 0 Yes | 2 No |
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