what is Sauton's media recipe?
- 2 Answers
- 13277 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Ingredients are
KH2PO4 0.5 g
MgSO4.7H2O 0.5 g
Citric acid 2.0 g
Ferric ammonium citrate 0.05 g
Glycerol 60 ml
Asparagine 4.0 g
Distilled water to 1000 ml.
Dissolve the reagents, except for the glycerol, in 300 ml
water using heat.
Once dissolved, add the glycerol and make up to 1 liter
with distilled water. Check pH and, if necessary, adjust to
pH7.4 with 1 M NaOH.
Autoclave at 121 oC for 15 min.
It has been reported that the addition of ZnSO4 (0.1 ml of
1% solution) and Triton WR1339 (5 ml of 5% solution)
improves growth of Mycobacterium. tuberculosis .
| Is This Answer Correct ? | 6 Yes | 0 No |
Answer / b.yazdanpanah
Ingredients are
KH2PO4 0.5 g
MgSO4.7H2O 0.5 g
Citric acid 2.0 g
Ferric ammonium citrate 0.05 g
Glycerol 60 ml
Asparagine 4.0 g
Distilled water to 1000 ml.
Dissolve asparagin separatly in 100 ml distilled water
using magnet stirer & heat upto 56 Deg C
After dissolve the other reagents, except for the glycerol,
in 300 ml water one by one,using magnet stirer , add the
asparagin solution and after 5 minuts add glycerol and then
make up to 1 liter.
with distilled water. Check pH and, if necessary, adjust to
pH =7.2 with 1 M NaOH or NH3 solution.
Autoclave at 121 oC for 15 min.
It has been reported that the addition of ZnSO4 (0.1 ml of
1% solution) improve growth of Mycobacteria.
| Is This Answer Correct ? | 0 Yes | 1 No |
Explain the importance of species of the molds Ustilago and Claviceps in medicine.
Why food is necessary?
How do you culture N. gonorrhea on what type of media?
HIV virus belongs to which family?
4 Answers Biological.e.limited,
diffrance between mould and yeast?
How the e.coli survives?
why the most probable number(MPN) technique is carried out?
What are plate hyperthermophiles ?
What is fecal coliform?
What is a terrestrial organism?
what is the use of ramachandran plot?
how does Mucus membrane protect the respiratory system?
Micro Biology 
