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Review your data from the entire module. Say we ask you to
redo the purification.Is there any step that you could
eliminate? Which one? Why can you eliminate it?
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Answer / gurudut
Yes, we could do either the DEAE or the affinity there is
no need to do both.
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Answer / vipul panchal
we can eliminate the steps that involves gradual precipitation or removal of irrelevant protein.
Once we found that out interest protein gets precipitate at this salt conc. or at this fraction we can eliminate the assay of each fraction.
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