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What is acid number?
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Answer / akash
It is the number of milligrams of koh required to neutralise
the free acids present in 1 gm of fat. The acid number tells
us the quantity of free fatty acid present in fat.
| Is This Answer Correct ? | 5 Yes | 0 No |
Answer / ram
the number of milligrams of koh required to neutralise
the free acids present in 1 gm of fat. The acid number tells
us the quantity of free fatty acid present in fat.The acid
number is also said to be POLENSKE number
| Is This Answer Correct ? | 3 Yes | 1 No |
Answer / sunilreddy.vancha
The acid number is also said to be POLENSKE number with the
same definition which is given above
| Is This Answer Correct ? | 2 Yes | 3 No |
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
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