Explain the Protein expression analysis?
Answer / guest
Protein microarrays and high throughput (HT) mass
spectrometry (MS) can provide a snapshot of the proteins
present in a biological sample. Bioinformatics is very much
involved in making sense of protein microarray and HT MS
data; the former approach faces similar problems as with
microarrays targeted at mRNA, the latter involves the
problem of matching large amounts of mass data against
predicted masses from protein sequence databases, and the
complicated statistical analysis of samples where multiple,
but incomplete peptides from each protein are detected
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Suppose you used the NCBI Blast server to look for a match to a fruit-fly gene. Suppose further that the best alignment contained this section of an alignment: Hit from database: XXXXXXXXXXXXXXNFSTSQ user sequence: SLEAEAAPASISPSNFSSSQ What do the Xs signify?
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Suppose you used the NCBI Blast server to look for a match to a fruit-fly gene. Suppose further that the best alignment contained this section of an alignment: Hit from database: XXXXXXXXXXXXXXNFSTSQ User sequence: SLEAEAAPASISPSNFSSSQ What do the Xs signify?
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