I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

2305

Name the fungus attracting ants and smelling very foul and rotten?

1995

what is NIH 3T3 transfection?

2193

Explain why the three-domain system was devised and identify the domains to which microorganisms belong?

2717

Garnet is the ore of which element?

1106

How test tube brush is used?

1044

What are the various filters?

1105

Explain about sacB gene - levan toxicity to gram-negative bacteria?

5884

How does the formation of the enzyme-substrate complex? Explain the reduction of the activation energy of chemical reactions?

1043

Give an account of antibodies against pET30c fusion protein ?

2207

What is the chorioallantois membrane present in the embryonic development of reptiles and birds?

1

Define what is the meaning of the term ‘lagomorpha’?

1233

7) Diabetes is recessive. A) If woman has diabetes and marries a man that doesn’t have it, is it possible for their offspring to acquire this disease? A A Aa Aa Aa Aa a a genotypes Phenotype 100% Aa, Heterozygous 100% No diabetes B) If none of the father’s ancestors had ever had diabetes, would their children have a chage of getting the disease? Explain

1920

Do enzymes act better under acid or basic ph?

1136

What are mitochondria? Why are they called as "power house" of a cell?

1086