What are the main signals used for gene finding in prokaryotic genomes? How are these signals introduced into the search algorithms?

What are the main approaches of predicting protein interactions using genomic context analysis?

What is the main idea of maximum parsimony in phylogenetic tree construction? What are the drawbacks?

Bootstrap analysis evaluates evolutionary trees by sampling columns from the original alignment with replacement (multiplying or removing some of them) and computing a proportion of times that a particular branch appears in the resulting trees. What is the main idea behind this approach?

Which of the following sequences contains the pattern [AG]-x (4)-G-K-[ST] from the PROSITE database? seq. A: VAGWGKST seq B: GVLKRGKS seq. C: AGVLKGRT seq. D: AGVGKSTP

If a match from a sequence database search is reported to have an E-value of 0.0, should it be considered highly insignificant or highly significant?

Suppose you used the NCBI Blast server to look for a match to a fruit-fly gene. Suppose further that the best alignment contained this section of an alignment: Hit from database: XXXXXXXXXXXXXXNFSTSQ user sequence: SLEAEAAPASISPSNFSSSQ What do the Xs signify?

Infosys,

How you caluculate sensitivity and selectivity of Blast?

You have been provided with a set of plates of fungi isolated from a diseased leaf, one Chytridiomycota, one Zygomycota, one Ascomycota and one Basidiomycota. The signs of infection indicate that the disease has been caused by a member of Ascomycota which does not form a sexual stage easily. What features would you look for in your isolates to indicate if the target fungus was present?

What are the hyphal features of Ascomycota?

In cleared and stained root material you have seen structures that appear to be empty sacs in the epidermal cells with ostioles that exit to the soil. Which division are these fungi likely to be from?

A sexual spore is formed in a culture from a single isolate. An attachment is found on either side of a sac- like structure, which encloses the spore. Which division does the fungus come from?

Toxin X is released only in mature spores of a single fungus and only when both alleles at a single locus are present. The toxin is never observed when the alleles are together in hyphae or modified hyphae. What division is the fungus found in?

TT,

What are the symptoms of Multiple sclerosis?

What does the Haversian (central) canal in each osteon contains?