How you will select column for HPLC development depends on
nature of compound and how it will work explain in brief?
Answers were Sorted based on User's Feedback
Answer / debananda mishra
Polar compounds can be analysed throgh polar stationary
phase and nonpolar compouds can separated through nonpolar
stationary phase but mobile phase used is vice versa.
Is This Answer Correct ? | 4 Yes | 1 No |
Answer / muzammil thange
Compounds should be seperated on "like likes like basis" ie polar compounds should be separated on polar columns and vice versa. Polar compounds when analysed on C-18 should be treated with modifiers to act like non-polar or neutral compounds.
Is This Answer Correct ? | 4 Yes | 2 No |
Answer / sarvesh pathak
1.if compound is polar select midpolar or towards the
nonpolar column e.g. C18 is midpolar column which is used.
2.if compound is nonpolar select polar stationary phase
e.g. silica column
3.look for the nature of compound whether it is ionisable
or non-ionisable
Is This Answer Correct ? | 4 Yes | 6 No |
Which products will produce when acetic anhydride reacts with Ter-butanol, Sec-butanol, Iso butanol, Dimethylamine etc.
What is Threshold in hplc?
How to caluculate the RRF and correction factor in hplc
1 Answers Alkem Labs, Aurobindo, Rakshit Drugs, SDLPL,
What is the use of Holmium (III) Oxide???
how to correct peak when peak comes broader in shape? How to do calibration of HPLC?
What is the meaning of 624 in DB-624 Capillary column used in GC?
how you determine moist.of NaOH sol or NaOH flacks
What is dose dumping? why require to do?
What is shaking level in GC?
The boiling point of 1,3 butadiene is -4.4 DC & the boiling point of Pentane is 36.1 DC. Then how come Pentane elute before 1,3 BD in GC analysis?
analytical method validation require to with respect to release specification or shelf life specification?
EXPLAIN THE CALCULATION PART OF AN KF TITRATOR CALIBRATION WITH SUITABLE EXAMPLE