how to correct peak when peak comes broader in shape?
How to do calibration of HPLC?
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Answer / satish
if peak is broad then use dilute solution of triethylamine to sharpen the peak
| Is This Answer Correct ? | 5 Yes | 1 No |
Answer / chanti1984
1. ENHANCE THE COLUMN OVEN TEMPARATURE, REDUCE THE SAMPLE
LOAD N COLUMN AND CHANGE THE DILUENT
2. URACIL/CAFFIENE SOLUTION ARE USING FOR CALIBRATION OF
HPLC WITH THEIR RESPECTIVE WAVE LENGTH
| Is This Answer Correct ? | 1 Yes | 2 No |
Why we don't add Hcl, HNO3, H3PO4 in residue on ignition, why we add H2SO4
if your impurity coeluting with each other in that situation how require to set specification? is it acceptable?
In BP2013, Loperamid HCl monograph. Assay by titration with 0.1N sodium hydroxide using hydrocloric acid 0.01N and reading the volume added between the 2 points of inflexion. I have a question that if the diluent solvent is ethanol is certainly consumed a amount of volume of titrant, so this volume must be eliminated on the result calculation or not apart from first point which is subtracted above.
What is the difference between the working standard and reference standard?
what is separation techniques.
Which detector is used in uv-vis spectrophotometer?
Why we are using a Kf Factor range 4 to 6
What is difference between chemical Assay and Hplc assay?
What is Impuriteis?
why octanol used to determine the partition coefficient ?
how do we get usp reference standard potency?
1 Answers Ind Swift Laboratories, MSN Pharma,
Difference between related substances & impurities
Organic Chemistry 
