iam using ph instrument lab india make.3ponit and 5point.in
calibration time 3point slope will come 98.89and 96.87.that
limit is 85 to 105.what basis slope will come.any
calucalation between them.5point wiicome slope 99.89?why
3p0int came 2 values
Answer / firoj m. mulani
It is simple,we know pH is inversally praportional to the Mv
ie If pH is Increases mv is decreases.
then,for calculating the slope at 5 point a simple formula
as below,
Mv1.68 - Mv4.01*100
---------------------
pH4.01 - pH1.68*59.16
Mv4.01 - Mv6.87*100
---------------------
pH6.87 - pH4.01*59.16
for next point's,formula as above
if 5 point pH it gives 4 values came of slope.
Is This Answer Correct ? | 1 Yes | 0 No |
what is peak purity? how may types of methods to determine peak purity?
what is the difference b/w repeatability nd reproducibility ?
what is end capping in hplc coulmn
2 Answers Lupin, Zydus Cadila,
why has inject 5 injection in assay
we calibrate HPLC once in 6 months while iam doing calibration i came to know that calibration was failed. then how can i justify that the samples analyzed on the system during this 6 months was wright? how can i forward in to the next step for those batches?
What is the difference between chromatographic purity and related substances?
what is the acceptance criteria for enteric coated tablets in 0.1n hcl validation in each parameter?
Why the same type of columns showing different RT for a single product?
How can we calculate "confidence interval" in analytical method validation? Pl. explain with example.
why we are using 6 dosage units in dissolution, and why we are using 6 tablets in disintegation test.is there any scientific reason beyond 6?
Why we are using a Kf Factor range 4 to 6
I want analyze my compound at 325 nm so which standard shall i use to calibrate my HPLC system? How i can assure my hplc detector is suitable or not?