how do start hplc method development?
how much sample required for developing the method
development?
- 3 Answers
- 51158 Views
- BMS, Cipla, Indoco Remedies, Sun Pharma, I also Faced
- E-Mail Answers
Answer Posted / kizar ahamed
Method development starts with solubility of the
compound,if itis soluble in water v can try with reverse
phase if is is not v should use normal phase
chromatography.
2)selection of wavelength
3) column selection
4)Gradient setting(Always develope the method by using
gradient than isocratic)
| Is This Answer Correct ? | 71 Yes | 25 No |
Post New Answer View All Answers
What is dose dumping? why require to do?
What is similarity factor and its use in api strength?
Why we use potassium dichromate in uv calibration Exact reason behind it??
how a particular wavelength can be different for a particular compund while analysing by uv and by HPLC.
how require to fix the linearity and accuracy cincentration range?
For limit test of heavy metals in BP, Method C require that the substance is ignited at a temperature not exceeding 800 °C. Why confines such the temperature?
How to choose the which salt is suitable for mobilephase
iam usig ph buffers merk. manually how to prepare ?
If we do accuracy at same concentration at which linearity planned,what is the need to do linearity separately?
Is it nessesary all multimedia dissolution require descriminatory?
Which parameter require to do for analytical method equivalency?
when we talk about change in entropy we consider total entropy means of surround +entropy of system but when we talk about change in Gibss free energy we consider entropy of system.explain with reason.
My question about gas chromatography sulfur chemiluminsecence detector. I test unknown sample gas by GC-SCD (calibrated ) and the result of *H2S is 279 PPM , *but when I test the same sample with the GC-TCD (calibrated ) the value of *H2S is 2500 PPM . I'd like to inform you that both GCs are calibrated and have very good operation conditions with stable parameters . the question is if the sample gas with higher H2S over detection limits of SCD detector (1000 ppm). why the result it 279 ppm Best regards
From where require to take the RLD sample?
What is the Formula for coreletion coefficient in plhplc calibration
