Topic :: Enzyme





Enzyme Interview Questions
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Which of the following enzymes secreted by pancreas helps predatory mammals to digest the blood they drink from their prey? 1 Pepsin 2 Fibrin 3 Trypsin 4 Ptyalin

2 5450

what are the disadvantages of immobilized enzymes

2 14386

What are advantages of using immolized enzymes?

6 6405

three advantages of immmobilized enzymes

1 3165

Name the two enzymes produced by phosphate solubilizing bacteria to solubilize complex phosphates.

3 5943

how to analyse serum cardiac marker, cardiac enzymes, troonin and myoglobin?

1954

what are the enzyme present in the saliva?

CBI,

8 8511

what is the advantage of immobilised enzyme in industrilized ?

Biocon,

1 6531

why is partial purification of enzyme done in some cases not complete purification ?

1 2816

Activity of an enzyme can be modulated by change of [a] pH [b] Light [c] Humidity [d] Rainfall

SSC,

1 6088

Question 56 - The kinetic behavior of an enzyme could be described using Michalis - Menten equation : Vo = Vmax [S] / (Km + [S]). Derive this equation from [ES] = [E]total [S] / (Km + [S]), Vmax = Kcat [E]total, Vo = Kcat [ES].

1 1847

BIOPROCESS ENGINEERING - EXAMPLE 14.3 : The kinetic behavior of an enzyme could be described using Michalis - Menten equation : Vo = Vmax [S] / (Km + [S]). Derive this equation from [ES] = [E]total [S] / (Km + [S]), Vmax = Kcat [E]total, Vo = Kcat [ES].

1 1840

In metabolism, enzymes act as ? a) Promoter b) Catalyst c) Oxidant d) Reluctant

1 944

The salivary glands secrete saliva which contains the enzyme ? a) Pepsin b) Renin c) Ptylin d) Lipase

1 1015

What is enzymes?

1 810




Un-Answered Questions { Enzyme }

Which vitamins participate, in coenzyme form, in reactions of the tricarboxylic acid cycle?

1705


A blastomere was removed from an 8-cell human embryo, and its beta-globin gene was amplified by PCR. The beta-globin DNA was treated with an enzyme that cuts on either side of and within the normal allele (producing a small fragment), but not within the sickle cell allele (producing a large fragment). Electrophoresis of the treated DNA from the blastomere demonstrated the presence of both a large and small fragment derived from the beta-globin genes. How should you interpret this test?

1584


Can angiotensin-converting enzyme (ACE) inhibitors and angiotensin receptor blockers cause urticarial skin eruptions?

2175


Compare and contrast feedback inhibition and enzyme repression?

9304


What are the enzymes produced by streptococci?

1663






The elution volume of an enzyme on a gel filtration column can be predicted from a) its enzyme activity b) its protein absorbance at 280 nm c) subunit composition and monomer molecular mass(es) d) Choices a) and b) are both correct.

2805


What is enzyme-ligand interaction?

1552


I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line

1723


how to analyse serum cardiac marker, cardiac enzymes, troonin and myoglobin?

1954


What are some biological examples in which lysosomic enzymes play fundamental role?

562


What are the enzymes used for labeling of antibodies?

174


Name two enzymes that have been employed for elisa?

203


What happen to a denaturated enzyme regarding its functionality? How that result can be explained with the help of the lock and key model?

665


What are allosteric enzymes?

606


What is the importance of enzymes for the living beings?

663