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what is the general mechanism of high performance liqiud
chromatography?
- 7 Answers
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Answer / swati
HPLC is partition chromatography, in which a solute(or
solutes)is partioned between two immiscible liquids, one of
them is mobile phase and other is stationary phase. The
granualar solid serves as amechanical support for the
stationary phase , as it requried that the two liq. phase
should be immiscible, they must be differ in degree of
polarity.
| Is This Answer Correct ? | 21 Yes | 11 No |
Answer / falguni patel
it is the saperation tech.in that if the system is reverse
phase (mobile phse-polar & stationary phase- non polar) &
sample is polar then polar component elute 1st with mobile
phase, less polar componantes elute later b'coz they
slightly adsorb on stationary phase, & so on
| Is This Answer Correct ? | 13 Yes | 4 No |
Answer / narasaiah aurobindo
main principle is adsorption. when mixture of componenets
passed through the column the components which are more
attraction with stationary phase eluted later and less
interaction components eluted first depend upon their
correlation coffecient and not two components having the
same interaction with column
| Is This Answer Correct ? | 8 Yes | 3 No |
Answer / nabil
Confusigly,there are two variants in use in HPLC depending
on the relative polarity of the solvent and the stationary
phase.
1-Normal Phase HPLC
The column is with tiny silica particles, and the solvent
is non polar hexane for example, Polar cpds in the mix
being passed through the column will stick longer to the
polar silica than the non polar cpds will, the non polar
ones will therefore pass more quickly through the column.
2-Reversed phase HPLC
In this case the silica is modified to make it non polar by
attacheing hydrocarbon chains to its surface typically with
either 8 or 18 carbon atomsin them. A polar solvent is used.
In this case there will bestrong attraction between polar
solvents and polar molecules will pass through
thecolumntherewon't be much attractoin between the
hydrocarbon chain and the polar solvent.
Non polar cpds in the mix will tend to form attractions
with the hydrocarbons gps because of van der waals forces
they will be also less soluble in the solvent because the
need to break the hydrogen bonds,they spend less time in
solution in the solvent, that mean the polar molecules that
will travel through the column more quickly.
| Is This Answer Correct ? | 4 Yes | 2 No |
In simple definition of HPL: The HPLC is the high performance liquid Chromatography, it is separation technique for components, it is working on two phases, first is mobile phase (moving phase) and second is stationary phase.
| Is This Answer Correct ? | 0 Yes | 0 No |
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