How we develop a method by HPLC for unknown compound,if we got three peaks with same response then how we can find out which one is our desired compound's peak without taking help of LC MS.
Answers were Sorted based on User's Feedback
Answer / dinesh solanki
If it is a assay method then inject std in hplc system and compare the chromatogram and identified the interested peak from them.
Same RC method inject std and identified the interested peak and other is may be impurities or contamination of sample.
Is This Answer Correct ? | 2 Yes | 1 No |
Answer / shashikant
We can interprit the peaks on the base of polarity of related impurities
Is This Answer Correct ? | 0 Yes | 1 No |
Answer / chennusrinivas
Based on drug Melting point we wil identity that particular
drug, from that will inject different concentration sample .
Is This Answer Correct ? | 3 Yes | 10 No |
Difference between standard operating procedure and standard test procedures in pharmaceutical industry
why we use sodium salicylate in uv light calibration at TLC chamber
1 Answers Aurobindo, Lee Pharma,
I have got selected for Aurobindo Pharma AR&D, HR person is asking me to sign in agreement. can I join?
process of Diclofenac sodium,IP.
How require to develop GC method? how to select diluent, GAS, column selection and other chromatographic conditions?
why you are using benzoic acid for water content determination in TEA
2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
which effect is mainly responsible for chromatographic separation when silica is the statinary phase at TLC? thanks in advance
Why base line not start from zero in GC Chromatography analysis.
why using the ph 4,7,9.2?
A new drug substance found fail to meet specification for an unknown Impurity during stability study(Specific change),how would further proceed?
Why karl fisher factor is 5 ?