What reagents can be used to break disulfide bonds?
- 10 Answers
- 28059 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / raghavendra naidu
1)DTT- Di thio tritol
2)BME- Beta mercapto ethanol
| Is This Answer Correct ? | 38 Yes | 0 No |
Answer / g.rajesh kumar
Mercaptoethanol breaks the disulphide bonds in proteins and
sds makes the protein to possess negative charge, so that
proteins can be separated based on their charge in sds-page
chromatography.
| Is This Answer Correct ? | 23 Yes | 1 No |
Answer / narayana
Di thio thretol,Beta marcapto ethanol.
Some times urine also.
| Is This Answer Correct ? | 2 Yes | 0 No |
Answer / g satheesh
sodium bisulfite in Base media or Zinc and acetic acid
| Is This Answer Correct ? | 3 Yes | 2 No |
Answer / kesava.sat@gmail.com
SDS pocess the proteins to positive charge and b-mercato ethanol degrades the disulphide bonds. and urea also degrades the disulphide bonds
| Is This Answer Correct ? | 1 Yes | 4 No |
Explain which branch of science deals with the transformation of chemical energy into electrical energy and vice versa?
What happen to a denaturated enzyme regarding its functionality?
Where is csf found? What is its function?
What is a plasmalogen? What do plasmalogens do?
What is meant by substrates of enzymatic reactions?
Define the phenomenon catenation. Which element has maximum catenation ability?
What is the phenomenon of Acrolein test?
In double bond, how many sigma and Pi bonds are present in it?
What is the difference between Gram positive & Gram negative bacteria based on its cell contents?
Explain kararch effect?
what is albinism?
Define bond angle?
Micro Biology 
