Difference Between End Cap HPLC Column and Non End Cap Column
- 2 Answers
- 13251 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / samar mandal
In end capped column the no of free silanol groups are very less compared to non end capped column.A capping reagent like Cl(CH3)2SiCH3 is used to bond with the sillica bed apart from C8 and C18 group, which eventually block the free silanol group by forming silyl ether(-Si-O-Si-), which decreases the reaction between analyte and free silanol groups.
| Is This Answer Correct ? | 37 Yes | 1 No |
Answer / sagar flamingo ltd
In Case of End Cap Column both end are connected with SS Cap Which is easily removable.But In Case of without End Cap Column there are not having such type adjustment.
| Is This Answer Correct ? | 2 Yes | 11 No |
Explain about Low pressure gradient and High Pressure gradient systems in HPLC?
4 Answers Mylan, Orchid, Roshanico,
a bottle of concerntrated HCL (36.5) was labelled as 37% (w/w) and density 1.19m/l.What is hcl molarity?
According to ICH,India is coming under which climatic zones?
why we cannot analysis below 200nm in u.v?
My question about gas chromatography sulfur chemiluminsecence detector. I test unknown sample gas by GC-SCD (calibrated ) and the result of *H2S is 279 PPM , *but when I test the same sample with the GC-TCD (calibrated ) the value of *H2S is 2500 PPM . I'd like to inform you that both GCs are calibrated and have very good operation conditions with stable parameters . the question is if the sample gas with higher H2S over detection limits of SCD detector (1000 ppm). why the result it 279 ppm Best regards
Why used 0.02% v/v solution of toluene in hexane for calibration of UV.
WHAT IS DIFFERENCE BETWEEN RRT AND RRF IN HPLC
what is SOP?How can prepare?
WHAT IS THE CRITERIA FOR SELECTION OF TIME OF DISSOLUTION AND THE MEDIUM OF DISSOLUTION?
why we use sodium salicylate in uv light calibration at TLC chamber
1 Answers Aurobindo, Lee Pharma,
what is the results if we use ODScolumn instead of BDS column ,(ther parameter is same for column) in assay method ?
What is difference between known and unknown impurities
Organic Chemistry 
