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What is colimn selection Criteria in HPLC analysis?
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Answer / m
Column selection is the most important part of your HPLC
method development
1 Choose the column according to the your column polarity
and pH range of buffer.
2 Mobile phase composition and what solvent you are used is
also depends
3 Carbon loading and surface area and micron size is also
depends if you are using more buffer concentraion (Aques)
for that hydro columns are also available
over all it depends on your compound and its polarity
| Is This Answer Correct ? | 21 Yes | 0 No |
Answer / eapen
Column selection depends on
1.HPLC Method adopted whether Normal phase or reverse phase.
2.Number of components present in the sample. if more
components present instead of short columns long columns
must be used.
3.The functional group present in the analyte.
Based on the functional group present we can use either
Amino,Cyano,Carbohydrate etc. columns.
4.Polarity of the components
eg. If a sample contains nonpolar components instead of
C18 column we can use C8 column or C4 columns.
| Is This Answer Correct ? | 14 Yes | 0 No |
Answer / ikhlakh
we can select hplc column mainly on the basis of analyte
nature i.e for acidic molecule acidic column,for basic
molecule basiccolumn.
| Is This Answer Correct ? | 8 Yes | 2 No |
Answer / amit
Depands on our analyte (Acidic or basic) select proper
mobile phase and on that basis can select our stationary phase.
If basic functional group, select acid buffer, and non polar
stationary phase (C18)
| Is This Answer Correct ? | 5 Yes | 1 No |
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