How to purify viruses?
Answers were Sorted based on User's Feedback
Answer / priya- engineer biotechnologis
Inverted ocmosis- in water, soluble strong polarised
molecule.
Is This Answer Correct ? | 0 Yes | 0 No |
Answer / bricy
The invention provides a method for the purification of a
virus from a host cell, the method comprising the steps of:
a) culturing host cells, b) infecting the host cells with a
virus, c) treating the cell culture with nuclease, d)
lysing the host cells to provide a lysate comprising the
virus. The virus is preferably a recombinant adenovirus.
The invention further provides a method for the
purification of a recombinant virus expressing a
heterologous protein that is capable of binding nucleic
acid, comprising the steps of: a) culturing host cells, b)
infecting the host cells with the recombinant virus, c)
lysing the host cells to provide a lysate comprising the
recombinant virus, d) subjecting the recombinant virus to
anion exchange chromatography and size exclusion
chromatography, characterized in that the virus-containing
mixture is buffer exchanged at least once with a solution
comprising at least 2 M NaCl, or another salt providing an
equivalent ionic strength. (end of abstract)
Is This Answer Correct ? | 0 Yes | 0 No |
Explain the major organs of the digestive system do?
Order the following from the part of the digestive tract nearest to the beginning to the part nearest to the end: jejunum, ileum, esophagus, duodenum?
What does CH2O represent?
Order the following from the largest taxonomic group to the smallest group: order, genus, kingdom, class, family, species?
Explain some common laboratory techniques?
What is a burette?
What are blood enzymes?
Explain the Gas chromatography ?
What are the uses of formycin b?
which insect doesn't drink water?
What is an erlenmeyer flask and how is it used?
WHAT DOES BADAM CONTAINS MINERAL OR PROTEIN NEED DETAIL ANSWER...