What is the superiority of TLC over paper chromatography?
- 9 Answers
- 38104 Views
- Ranbaxy, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / ravi
ADVANTAGES OF TLC OVER PAPER
1. high speed
2. high sensitivity
3. small sample required
4. sharper preparation of sample
5. various reagents are used without damaging plates
6. large choice of adsorbents
7. after development , sample easily separated
| Is This Answer Correct ? | 79 Yes | 3 No |
Answer / kavitha jai
when compare to paper chrmatograpy TLC having more
advantage because in TLC we are having wide joice of
adsorbent depend upon our sample and development. after
development sample can seperate easily from TLC. higher
version of TLC like HPTLC and more type is their for
purification of the sample.
| Is This Answer Correct ? | 29 Yes | 8 No |
Answer / s.sindhu
the superioity of TLC over paper chromatography is TLC
requires less time for separation of aminoacids,plant
pigments etc
| Is This Answer Correct ? | 24 Yes | 5 No |
The superiority of TLC over paper chromatography
because of inorganic nature of adsorbent concentrated
sulfuric acid spray followed by heating may be used to
develope on the chromatogram by charring.
moreover aminoacid mixtures which require 18 hours for
seperation on paper require 3 hrs using cellulose TLC.
advantage also lies in choice of adsobents which allow
seperation not possible on paper.
| Is This Answer Correct ? | 21 Yes | 15 No |
Answer / victoria
In tlc the stationary phase is a silica gel plate vt a supprting medium wher asin papr the sstationary phase is d water present in cellulose. More crrosive reagents can b used in tlc eg. Sulfuric acid, wher as corrosive rgnts cn spoil d stationary phse in paper chromatography.. tlc is a faster less time consuming technique n seperation of d spots is also faster.. most importantly the proper sepearration of d spots in tlc is due to d particle size o f d stationary phase.. here the partcles are evenly packed n siz is smaller ..
| Is This Answer Correct ? | 4 Yes | 0 No |
Answer / swadhin ghosh
1) Higher sample throughput and lower analysis time, as the number of samples that can be separated on one plate in TLC, is far more than the number of samples that can be separated in one paper.
2)Strong reagents can be used for the purpose of identification, as the TLC plate is able to withstand strong solvents and colour-forming agents.
3)TLC plates can be heated to higher temperatures(for spot development) without any damage, which might not be possible in case of PC, lest the paper gets damaged.
4)The sensitivity of detection is more in TLC, as compared to PC, because of lesser spreading of substance zones, in a TLC plate, than what occurs in PC.
| Is This Answer Correct ? | 3 Yes | 0 No |
Answer / syeda humaira
TLC is superior over paper in most of the ways but the most
important one is,on TLC plate corrosive reagents can be
used for identification of spots but in paper it gets
charred and the sample spot gets destroyed
| Is This Answer Correct ? | 12 Yes | 11 No |
Answer / shafira
InTIC,silica gel acts as a stationary phase.
In PC, water present in the cell use of paper is stationary phase
| Is This Answer Correct ? | 2 Yes | 3 No |
Answer / gayatri
in tlc silica gel is used as ststionary phase. in paper
cromatography paper itself is used as a stationary phase
| Is This Answer Correct ? | 7 Yes | 10 No |
if there is solution , how will u estimate that it contains DNA only, not RNA? how we differentiate DNA ,RNA and Protein in solution without studying its structure?
What is the difference between SDS Page gel electrophoresis and native gel?
What should be included on the gel to enable you to determine actual sizes of the DNA fragments?
What are immunoglobulins?
I will be attending an interview conducted by BCIL (Biotech consortium India Limited) in 2 days. Can someone who has attended the interview in the previous years help me regarding the kind of questions asked?
what is differance between trans acting element and transcription factor?
what happen when there is absence of sulfur cantaining amino acid in protein synthesis?
How is this bio technology useful in our day to day life?
is the blood sterile or non- sterile ?
What is necessary for commercial production of a biotech plant?
what are your strenths & weaknesses
what is biological hydrogen production? 0 7 Hai friends, Can someone guide me..Am a postgraduate(m.tech) in biotechnology and am thinking of doing pg diploma course through distance mode. are they worth doing? if so which is better pg diploma in bioinfo or pg diploma in Intellectual property rights..am awaiting ur suggestions 0 20 hey i am doin bsc biotech and i want to do research on stem cells.....plz tell me how to go about?????? 0 32 in the lcmv what type of effect onm the mhc class i molecule 0 18 WHAT IS MEAN BY GENETIC ALGORITHM 1 56 Can I get a working model on petro plants ? 0 76 if i m doing bsc in biotechnology now.. are there any chances that i can do b.tech nw?? 0 59 what is multicolour biotechnology? what are the applications of multicolour biotechnology? 4 275 will i get f1 visa to pursue masters in US, if i have many backlogs and more than 2 yrs gap in btn my graduation. 0 85 suppose if u have gone through any biotech industry, what are the environmental conditions that u r expecting in quality control? Shanta-Biotech 2 598 suppose if u have gone through any biotech industry, what are the environmental conditions that u r expecting? Shanta-Biotech 1 261 what is the process of chees preparation 1 240 how biodiesel helps in reducing co2 1 313 what is multi colour bio technology? 1 200 i m doing bsc in biotechnology n i m confuse what to do in future...whether i shall do msc in biotechnology or any otyer course?in future which field will have greater oppurtunity?
Micro Biology 
