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what percentage of agarose is required to load 2kb dna
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Answer / dixon davis
1%. 0.8% gel will show good separation (resolution) of large DNA fragments (5–10 kb) and a 2% gel will show good resolution for small fragments (0.2–1 kb).1% will be ideal for 2kb DNA.
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Answer / soumyendu(jeet) ghosh
For the given size of DNA(2kb), about 1%W/v of agarose gel will suit the most for a good resolution.
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Answer / priyanka
The volume of agarose required for a minigel is around 30–50
mL, for a larger .
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Answer / vivega
Linear dsDNA Size Range (base pairs) Agarose Concentration (% w/v)
1,000 - 23,000 0.6%
800 - 10,000 0.8%
400 - 8,000 1.0%
300 - 7,000 1.2%
200 - 4,000 1.5%
100 - 3,000 2.0%
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Answer / daisy masih
it is 2 percent agarose concentration used for 2 kb DNA
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