why use toline in resolution in uv calibration
- 1 Answers
- 6311 Views
- Reddy Labs, I also Faced
- E-Mail Answers
Answer Posted / n.srikanth
The Spectral Bandwidth (SBW) of a spectrophotometer is the
basis of establishing its ability to resolve absorbtion
lines seperated by small differences in wavelength. If a
substance that you need to measure has an absorbtion line at
257.4nm and there is an interfering line at 260.0 nm you
need to establish whether your spectrophotometer can resolve
the two lines seperately or "mixes" the two absorbtion lines
into a single line which would give erroneous results.
Toluene in Hexane is used as a reference for the calculation
of SBW. The ratio of the absorbtion of the solution when
read at a maxima (around 268.7nm) and at a minima (around
267.0nm) relates directly to the SBW of the instrument being
assessed. Regular use of this technique will assure that the
resolution of your instrument is within the required range
for your work
| Is This Answer Correct ? | 4 Yes | 2 No |
Post New Answer View All Answers
what is the mechanism of action of local anaesthetics?
pls send me last two years solved papers of health & malaria inspector to kumar.santosh087@yahoo.com
why nitrogen charecter as a inert gas plz identified
How can I assign a retest date for solvents e.g. toluene, methanol, etc?
Give an example of how you've handled a problem patient.
What are the filing requirements for ANDA-USA, Europe, Brazil, South Africa, Japan and Emerging Markets
Tell us about a time when you failed to meet a deadline. What were the repercussions?
What aspect of your communications skills have you improved the most in and how did you accomplish this improvement?
How to preform dissolution profiling
what is the leak test procedure of filled bottles (liquid)
What is forced degradation study?
Tell us about your oncology experience.
Tell us about a situation in which you took the extra step for a patient.
what is your experience in medical continuing education.
What is the role of buffers (ex: KH2PO4) in HPLC analysis,
