what are the principles involved in thin layer
chromatography?
- 12 Answers
- 38699 Views
- Aurobindo, Reddy Labs, I also Faced
- E-Mail Answers
Answer Posted / kenne hippolyte
Similar to other chromatographic methods TLC is also based
on the principle of separation. The separation depends on
the relative affinity of compounds towards stationary and
mobile phase. The compounds under the influence of mobile
phase (driven by capillary action) travel over the surface
of stationary phase. During this movement the compounds with
higher affinity to stationary phase travel slowly while the
others travel faster. Thus separation of components in the
mixture is achieved.
Once separation occurs individual components are visualized
as spots at respective level of travel on the plate. Their
nature or character are identified by means of suitable
detection techniques.
| Is This Answer Correct ? | 3 Yes | 0 No |
Post New Answer View All Answers
can i used antimony potassium tartrate against potassium tartrate in Fehling B solution ?
Which theory explains the paramagnetic nature of oxygen? Who proposed it?
Define the phenomenon resonance?
What amount of catalyst is consumed in the reaction it catalyzes?
How are free energy, equilibrium and spontaneity related to each other?
What is addition reaction?
I have given the protocol for the cyclodextrin glygosyl transferase assay: One ml of appropriately diluted enzyme sample was incubated at 60 °C for 15 min with 5 ml of 1% (w/v) gelatinized soluble starch in 50 mM, 7.0-pH Tris–HCl buffer. Reaction was terminated by boiling the reaction mixture for 3 min and reaction volume was made to 10 ml with distilled water. Two ml of above reaction mixture was withdrawn and mixed with 3 ml of Tris–HCl buffer, 5 ml of 125 mM Na2CO3, and 0.5 ml of phenolphthalein (25 mg phenolphthalein/100 ml absolute alcohol). Absorbance was measured at 550 nm. The percent decrease of sample was calculated with respect to control containing 5 ml of buffer, 5 ml of sodium carbonate and 0.5 ml of phenolphthalein. where Acontrol = absorbance of control and Atest = absorbance of sample. The amount of β-cyclodextrin (β-CD) produced was estimated from the standard graph of 0–500 μg/ml β-CD concentration against % decrease in absorbance. One unit of CGTase was defined as the amount of enzyme required to produce 1 μm of β-CD/min. Please can you suggest me the formula for the defination given in the last line
What are similarities and differences between the transcription process and the replication processes?
give the co-ordination number of nacl?
Garnet is the ore of which element?
Define the phenomenon catenation. Which element has maximum catenation ability?
What is activation energy?
Hi, I am venkat. I am done with M.Sc Medical Biochemistry in the year 2007. I got rejected 3 times at US consulate, and the reason they say is why are you going agian for PG when you have already done PG, and they are not ready to get convinced only on this issue.
Where can rna are found within cells?
Aromatic compounds generally undergo which type of reactions?
