Answer Posted / biomaster
1) Fix smear by heat.
2) Cover with crystal violet.
3) Wash with water
4) Cover with gram’s iodine
5) Wash with water. do not blot.
6) Decolorize for 10-30 sec with gentle agitation in
acetone(30ml)and alcohol(70ml).
7) Wash with water. do not blot.
8) Cover for 10-30 sec with safranin(2.5% solution in
95% alcohol.)
9) Wash with water and let dry.
| Is This Answer Correct ? | 7 Yes | 2 No |
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