what is the peak purity concept?
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Answer Posted / raghavaiah mandalapu
A technique is described whereby the purity of an HPLC peak
can be determined by using electrospray liquid
chromatography-electrospray ionization mass spectrometry.
Electrospray mass spectra acquired across an HPLC peak are
summed and examined for co-eluting impurities. The mass
spectrometer is set up to produce solely cationised
molecules and background noise is minimized so that minor
coeluting impurities can be observed down to a level of
<0.1% of the major component. This method offers advantages
over using diode-array UV detection (LC-DAD) for the
determination of HPLC peak purity, namely components with
similar UV spectra can be distinguished, the molecular mass
of the impurity can be determined and structural data can
be obtained by using tandem mass spectrometry (MS-MS). The
effectiveness of the technique is demonstrated with drug
substances of pharmaceutical interest which have been
chromatographed on an HPLC system designed to intentionally
co-elute a number of impurity standards with these
compounds.
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