Suppose you used the NCBI Blast server to look for a match
to a fruit-fly gene.

Suppose further that the best alignment contained this
section of an alignment:

Hit from database: XXXXXXXXXXXXXXNFSTSQ

user sequence: SLEAEAAPASISPSNFSSSQ
What do the Xs signify?

Answer Posted / swapnil

that x may be any amino acid and we can consider any value
of identity on the basis of matching of user seq with hit

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