Answer / kangchuentat

ENVIRONMENTAL ENGINEERING - ANSWER 22.3 : (a) Flowrate = (225 US-gallon / minute) / [ (hour / 60 minute) x (day / 24 hour) ] = 324000 or 0.324 MGD. (b) Chlorine feed rate = 0.324 MGD x 2 mg / L x 8.34 = 5.40432 lb / day. (c) The constant is derived from 0.0000022046 pound / mg x 1000000 US-gallon / M US-gallon x 3.78541 L / US-gallon = 8.345 with minor error (proven). The answer is given by Kang Chuen Tat; PO Box 6263, Dandenong, Victoria VIC 3175, Australia; SMS +61405421706; chuentat@hotmail.com; http://kangchuentat.wordpress.com.

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MICROBIOLOGICAL ENGINEERING - QUESTION 28.2 : A hemocytometer is a device that is used for counting cells. In an engineering experiment, 100 microlitres of cell suspension is diluted with 50 microlitres of Trypan blue dye. Only death cells appear blue in color when stained with the dye. There are 57 cells detected in a hemocytometer, where 5.3 % of them appear blue when the chamber of the meter is placed under a microscope. Each square of a chamber can contain 0.0001 mL of liquid. (a) Calculate the number of viable cells. (b) The cells occupied 5 squares. Calculate the average number of viable cells / square. (c) Calculate the dilution factor of the cell suspension by using the formula : Dilution = final volume / initial volume. (d) Calculate the concentration of viable cells / mL by using the formula : Concentration = (Average number of viable cells / square) x dilution x (square / volume).

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Question 72 - (a) According to United States Department of Agriculture (USDA) (http://ndb.nal.usda.gov/ndb/search/list, accessed 12 August 2016), 100 g of potatoes generate 77 kcal of energy. For raw tomatoes, 111 g have 18 kcal of energy. Question : How much energy will one gain if 150 g of heated potatoes are eaten with 200 g of raw tomatoes? (b) If 1 Calorie = 1 food Calorie = 1 kilocalorie and 1000 calories = 1 food Calorie, then how many Calories are there in 9600 calories? (c) According to a food package of potato chips, 210 Calories are produced per serving size of 34 g. In actual experiment of food calorimetry lab, 1.75 g of potato chips, when burnt, will produce 9.6 Calories. For each serving size of potato chip, find the difference of Calories between the actual experimental value and the value stated on the food package. (d) The specific heat of water is c = 1 cal / (g.K) where cal is calory, g is gram and K is Kelvin. Then what is the temperature rise of water, in degree Celsius, when 150 g of water is heated by 9600 calories of burning food?

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Do you have any idea on recombinant protein expression?

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