Golgappa.net | Golgappa.org | BagIndia.net | BodyIndia.Com | CabIndia.net | CarsBikes.net | CarsBikes.org | CashIndia.net | ConsumerIndia.net | CookingIndia.net | DataIndia.net | DealIndia.net | EmailIndia.net | FirstTablet.com | FirstTourist.com | ForsaleIndia.net | IndiaBody.Com | IndiaCab.net | IndiaCash.net | IndiaModel.net | KidForum.net | OfficeIndia.net | PaysIndia.com | RestaurantIndia.net | RestaurantsIndia.net | SaleForum.net | SellForum.net | SoldIndia.com | StarIndia.net | TomatoCab.com | TomatoCabs.com | TownIndia.com
Interested to Buy Any Domain ? << Click Here >> for more details...


Question 89 - A hemocytometer is a device that is used for counting cells. In an engineering experiment, 100 microlitres of cell suspension is diluted with 50 microlitres of Trypan blue dye. Only death cells appear blue in color when stained with the dye. There are 57 cells detected in a hemocytometer, where 5.3 % of them appear blue when the chamber of the meter is placed under a microscope. Each square of a chamber can contain 0.0001 mL of liquid. (a) Calculate the number of viable cells. (b) The cells ocupied 5 squares. Calculate the average number of viable cells / square. (c) Calculate the dilution factor of the cell suspension by using the formula : Dilution = final volume / initial volume. (d) Calculate the concentration of viable cells / mL by using the formula : Concentration = (Average number of viable cells / square) x dilution x (square / volume).



Question 89 - A hemocytometer is a device that is used for counting cells. In an engineering experi..

Answer / kangchuentat

Answer 89 - (a) Number of death cells = Total cells x % of death cells / 100 = 57 x 5.3 / 100 = 3.021 rounded to 3 cells. Number of viable cells = Total cells - number of death cells = 57 - 3 = 54 cells. (b) Average number of viable cells / square = 54 / 5 = 10.8 cells / square. (c) Dilution = final volume / initial volume = (100 + 50) microlitres / 100 microlitres = 1.5. (d) Concentration = (Average number of viable cells / square) x dilution x (square / volume) = 10.8 cells x 1.5 x (1 / 0.0001 mL) = 162000 cells / mL. The answer is given by Kang Chuen Tat; PO Box 6263, Dandenong, Victoria VIC 3175, Australia; SMS +61405421706; chuentat@hotmail.com; http://kangchuentat.wordpress.com.

Is This Answer Correct ?    0 Yes 0 No

Post New Answer

More Chemical Engineering Interview Questions

What is the purpose of capacitor?

0 Answers  


What is some good advice for specifying allowable pressure drops in shell and tube exchangers for heavy hydrocarbons?

0 Answers  


Question 45 - According to Raoult’s law for ideal liquid, x (PSAT) = yP where x is mole fraction of component in liquid, y is mole fraction of component in vapor, P is overall pressure and PSAT is saturation pressure. A liquid with 60 mole % component 1 and 40 mole % component 2 is flashed to 1210 kPa. The saturation pressure for component 1 is ln (PSAT) = 15 - 3010 / (T + 250) and for component 2 is ln (PSAT) = 14 - 2700 / (T + 205) where PSAT is in kPa and T is in degree Celsius. By assuming the liquid is ideal, calculate (a) the fraction of the effluent that is liquid; (b) the compositions of the liquid and vapor phases. The outlet T is 150 degree Celsius.

1 Answers  


Are some heat transfer services more prone to tube vibration that others for a shell and tube exchanger?

0 Answers  


Chemical Engineering Energy Balance - Please match the term A - E as follow with the stated definition : A. Yield. B. Selectivity. C. Relative saturation. D. Molal saturation. E. Absolute saturation. i. Answer : ? (moles of desired product formed) / (moles that would have been formed if there were no side reactions and the limiting reactant has reacted completely). ii. Answer : ? (moles of desired product formed) / (moles of undesired product formed). iii. Answer : ? (relative humidity 40 % means partial pressure of water vapour equals 4 / 10 of the vapour pressure of water at the system temperature). iv. Answer : ? (moles of vapour) / (moles of vapour dry gas). v. Answer : ? (mass of vapour) / (mass of dry gas).

1 Answers  


Question 10 - In the US (United States), one barrel is equal to 42 US gallons and equivalent to 0.15898 cubic metres. One British barrel is equal to 36 Imperial gallons and equivalent to 0.163659 cubic metres. If an oil refinery in England sells 42 Imperial gallons of crude oil to a company in US, how much US gallons of crude oils has been sold?

1 Answers  


hai can anyone send me soft copy of o.p gupta or tell me downloading link please please at saify_0@hotmail.com

0 Answers  


How many grams per liter would there be in a 0.35 n (normality) citric acid solution?

0 Answers  


How many years you are having experience in supervising others in lab environment?

0 Answers  


What are the some common causes of control valve noise?

0 Answers  


ACCOUNTING AND FINANCIAL ENGINEERING - EXAMPLE 34.32 : In the calculation of the total cost of production of an engineering material, a linear equation Y = MX + C could be applied. Let T = total cost, F = fixed cost, V = variable cost, Q = quantity produced, W = variable cost per unit produced. Form a linear equation that relates : (a) T, F and V; (b) V, Q and W.

1 Answers  


what is fund flow definition

1 Answers   Amara Raja,


Categories
  • Civil Engineering Interview Questions Civil Engineering (5086)
  • Mechanical Engineering Interview Questions Mechanical Engineering (4456)
  • Electrical Engineering Interview Questions Electrical Engineering (16639)
  • Electronics Communications Interview Questions Electronics Communications (3918)
  • Chemical Engineering Interview Questions Chemical Engineering (1095)
  • Aeronautical Engineering Interview Questions Aeronautical Engineering (239)
  • Bio Engineering Interview Questions Bio Engineering (96)
  • Metallurgy Interview Questions Metallurgy (361)
  • Industrial Engineering Interview Questions Industrial Engineering (259)
  • Instrumentation Interview Questions Instrumentation (3014)
  • Automobile Engineering Interview Questions Automobile Engineering (332)
  • Mechatronics Engineering Interview Questions Mechatronics Engineering (97)
  • Marine Engineering Interview Questions Marine Engineering (124)
  • Power Plant Engineering Interview Questions Power Plant Engineering (172)
  • Textile Engineering Interview Questions Textile Engineering (575)
  • Production Engineering Interview Questions Production Engineering (25)
  • Satellite Systems Engineering Interview Questions Satellite Systems Engineering (106)
  • Engineering AllOther Interview Questions Engineering AllOther (1379)