why we inject sample TWICE in ASSAY by HPLC analysis but in RS once?

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why we inject sample TWICE in ASSAY by HPLC analysis but in RS once?..

Answer / raveendra kumar

we injecting the two injections from one sample preparation to know the precision and reputability of the analyte.
(During real analytical practices we inject only two injection in single assay analysis. So accuracy of single injection is question.)

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why we inject sample TWICE in ASSAY by HPLC analysis but in RS once?..

Answer / krishnakumar

RS is qualitative nd assay is quantitative analysis.for that purpose nd for get better accuracy,we r prepare two preparations and injected two injections in assay analysis.

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why we inject sample TWICE in ASSAY by HPLC analysis but in RS once?..

Answer / dr.r.navaneeswri

We inject good average result coming or not in rs now a days 2injections or two preparations injections are injected

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5 Yes

5 No

why we inject sample TWICE in ASSAY by HPLC analysis but in RS once?..

Answer / abdul khadar shaik

Related compounds:Generally,We are using two preparation and two injections by HPLC and GC.After compleation of Related compounds validation,we are using one preparation and one injection.

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