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why we get only one peak in assay by hplc but in rs by hplc get more peaks
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Answer / k kumar nagendra
In assay by HPLC only the analyte peak is required to calculate againest the standard peak of the sample compuond hence the other impurity peaks are not required so sample in dilute to surtain level to identify the only major single peak hence we get single peak in assay by HPLC.
In some cases both the assay and impurities can be determine with the single method.
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Organic Chemistry 
