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What is the difference of time between two consecutive injections in HPLC?
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Answer / fionahu
There are several reasons for the time difference between two consecutive injections in HPLC. Based on Van Deemter equation, the plate height is dependent on the linear velocity of the mobile phase , multiple path effects ( eddy diffusion), longitudinal diffusion, and mass transfer between mobile phase and stationary phase. All of these factors above could result in peak broadening in LC chromatogram, which shows as elution time differences between different injection of the same samples.
| Is This Answer Correct ? | 1 Yes | 1 No |
theare are two consecutive injcetors in HPLC. auto
injection, manual injection.
in auto injcection the samples are placed in tray its
having 100 above vials holes, where that saples are placed.
in manual the samples are injected buy syrangy in vials
| Is This Answer Correct ? | 0 Yes | 11 No |
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My question about gas chromatography sulfur chemiluminsecence detector. I test unknown sample gas by GC-SCD (calibrated ) and the result of *H2S is 279 PPM , *but when I test the same sample with the GC-TCD (calibrated ) the value of *H2S is 2500 PPM . I'd like to inform you that both GCs are calibrated and have very good operation conditions with stable parameters . the question is if the sample gas with higher H2S over detection limits of SCD detector (1000 ppm). why the result it 279 ppm Best regards
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