How polarimeter is calibrated?
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Answer / rambabu vattikuti
Polarimeter calibration use below standards in different concentrations.
Compound Name value in [α]D (in o)
(1R)-(+)-Camphor +44.26
Sucrose +66.47
Cholesterol -31.50
D-(+)-Glucose +52.70
D-(-)-Fructose -92.00
Morphine -132.00
L-Proline (in water) -84.00
use above standard for calibration purpose .
1) Linearity
2) precision
parameters respective wave length
II) Polarity cell use wave length accuracy purpose
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