What is use of LOD and LOQ in validtion and give range and in calculation why we

What is use of LOD and LOQ in validtion and give range and in calculation why we use 3.3* LOD here why we use 3.3

Question Posted / shivashankaer_aravini

2 Answers
6869 Views
Dr Reddys, IPCA, Reddy Labs, I also Faced
E-Mail Answers

Answers were Sorted based on User's Feedback

What is use of LOD and LOQ in validtion and give range and in calculation why we use 3.3* LOD here w..

LOD is Limit of detection. We can dedect the peak but cant quantify
LOQ Limit od quaantification. We quantify also this is known as reporting thereshold.
During the RS analysis Below LOQ level we cant included in the total impurities calculation.
LOD and LOQ Determination three methods
1.S/N Ratio method
3 or 2 ratio for LOD
NLT 10 ratio for LOQ

2. Slope Method
Plot the linearity for active and impurities through serial dilution at lower level
Formula for LOD
3.3*Slope/Std.dev
Formula for LOQ
10*Slope/Std.Dev
3. Visualization method
It will be using for only identification and TLC Method

Is This Answer Correct ?

1 Yes

0 No

What is use of LOD and LOQ in validtion and give range and in calculation why we use 3.3* LOD here w..

Answer / kovida

LOD and LOQ are the smallest amount of the particular compound that can by detected and quantiified by using the developed HPLC method.

The signal to noise ratio is that minimum amount which when injected in HPLC it gives minimum detectable peak area.

The value of amount at this point is multiplied by 3 to get LOD
and by 10 to get LOQ value. In short LOD is the thrice value of minimum value and LOQ is ten times of minimum value that can be injected in HPL

Is This Answer Correct ?

1 Yes

2 No

Post New Answer

More Analytical Chemistry Interview Questions

Difference between related substances & impurities

2 Answers Biocon,

what is mean by nitrosamine impurities?

0 Answers

why we use vanillin in melting range calibration

3 Answers

wat is reverse phase in hplc

2 Answers

in dissolution why pool sample needed? in which type of drug pool sample need?

0 Answers Torrent Pharma,

What is the lab normality??

3 Answers DRL, Tagros,

WHAT IS DIFFERENCE B/W MALTINGRANG/DSC

1 Answers Glochem,

How you collect dissolution media while in stirring condition of a basket or a paddle?

2 Answers Mylan,

In kf constant term 16 what it is explain ?

2 Answers

USP methodology, EP methodology, IP methodology, among three if possible to use one methodology for qualify working standard to use USP, EP, IP ? Please explain...

0 Answers Srini Pharmaceuticals,

why in hplc peaks are in positive side(it means upside) not in negative side?

3 Answers

why we make titiration of Iron with EDTA ?

1 Answers Cipla, Sitec Labs,

For more Analytical Chemistry Interview Questions Click Here