How to know HPLC column performance?
Answers were Sorted based on User's Feedback
Answer / vijay muley
1) check the back pressure,
2) check Theoretical plates,
3) system suitability as per test requirement e.g. tailing
factor, symmetry, RRT, resolution etc.
all three parameters shows columns performance.
Is This Answer Correct ? | 20 Yes | 0 No |
Answer / darose
check for the number of plates that it maintains stady all
over the run , check the SST at avery run that cpmplies the
acceptance criterias of the method.
Is This Answer Correct ? | 5 Yes | 2 No |
Answer / venkat reddy
checked the column ressure
check theoretical plates
asymmentry RRT Resolution tailing factor compound method analysis
Is This Answer Correct ? | 0 Yes | 0 No |
Answer / deepak
To check theoretical plates,peak shape and resolution means sst of method.
Is This Answer Correct ? | 0 Yes | 0 No |
What should be the precision for HPLC during technology transfer?
in calibration of disintegration test apparatus why cycle limit is 28-32?
Why karl fisher factor is 5 ?
what is the analytical procedure for COD and BoD
reasons for negative peaks in chromatography
what is LC - MS ? What its wide use in cro/pharmaceutical Industry
why we multiply 1000 in the determination of kf factor ?
3 Answers Stellence Pharm Science,
What is the difference between GSC & GLC?
A new drug substance found fail to meet specification for an unknown Impurity during stability study(Specific change),how would further proceed?
wat is reverse phase in hplc
how to calculate elemental impurities?
If we have 5 strength which is not dose proportinate and excipients also diffrent in each strength then how we can proceed for Force degradation? and excipient are same but not dose propotinate the how FD?