Explain in detail what are the procedure and calculations
involved for accuracy (%Recovery)in method validation?
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Amount recovered (µg)
% Recovery = -------------------------X 100
Actual amount added (µg)
AreaSampleX Wt.Std X Potency
Amount recovered(µg)= ----------------------------
Area of Standard x 100
Amount added (µg) X Potency
Actual amount added(µg)= ----------------------------
100
Weight of std taken for recovery stock(mg)
( X Recovery Stock Solution addaed (ml)X1000
Amount added(µg)= ---------------------------------------
10
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