Why noise test is performed at 254 nm in calibration of HPLC
system?
Answers were Sorted based on User's Feedback
Answer / paresh jobanputtra, piramal
Earlier fixed wavelength detector is mostly used i.e 254 nm
because almost all Benzene ring containing compounds absorbs uv light
at 254 nm. i think due to this reasons only.
Regards,
Paresh Jobanputtra
Is This Answer Correct ? | 18 Yes | 1 No |
Answer / tejas shah
At 254nm none of the solvent or its impurities show significant interference so the noise will be recorded of pure UV light. i.e. detector noise
Is This Answer Correct ? | 0 Yes | 0 No |
Answer / jayesh patel
Generally all organic compounds have absorption at this
wavwlength.
Is This Answer Correct ? | 3 Yes | 6 No |
Why we are using the methanol in Kf titration?
what is rt and rrt? how we find rrt? relation between rt and rrt/
what is partion and column chromatography
What are the primary standards?
What is the exact reason to use THF and IPA in buffers of Reverse phase HPLC as a solvent?
which chromatographic technique is used for softening hard water?
what is difference between assayand potency
how to prepare solution in ppb
in sampling of raw material which side is pasted sampled labels
Which column is more polar, C8 or C18?
6 Answers Cipla, Glenmark, Laurus, Torrent Power,
why require to do water content for drug product?
WHAT IS THE USE OF STARTER ?????????