how to hplc calibrated
Answers were Sorted based on User's Feedback
HPLC is calibrated by the following parameters:
1) Pump calibration
2) Detector Linearity
3)Injector Precision and carryover test
4) Wavelength accuracy by using caffeine and pyrene solution
5) Determine the column oven temp. and sample compartment temp
6) Gradient test
7) Detector noise
Is This Answer Correct ? | 128 Yes | 13 No |
Answer / ajinkya p.
In HPLC there must calibrations for Pump, Detector,injector
Pump :- Should calibrated by checking flow accuracy test, Pressure holding test.
Detector :- Should calibrated by checking Linearity test, Detector wavelength accuracy test
Auto sampler :- Should calibrated by Injector Accuracy test,
Carryover test, Injector precision test, carry over test
Is This Answer Correct ? | 48 Yes | 2 No |
Answer / kanakadurga
calibration parameters are
1.Flow accuracy
2Temperature accuracy
3Wave length accuracy by using caffeine and erbium perchlorate
4Linearity of detector and injector
5.Carry over test
6.Drift and noice
Is This Answer Correct ? | 37 Yes | 6 No |
Answer / b.ganesh
calibration parameters are
1.Flow accuracy
2Temperature accuracy
3Wave length accuracy by using caffeine and erbium perchlorate
4Linearity of detector and injector
5.Carry over test
6.Drift and noice
7.Gradient progromme accuracy
8.injector precision
Is This Answer Correct ? | 3 Yes | 1 No |
what is mean by covalidation
Do you have experience with organic extractions or tissue digestions?
What is method validation in quality control
What is dose dumping? why require to do?
why 0.1M OR 1M Perchloric acid standardisation done after 24 hours?
How to estimate potassium?
If we change lenght of ph eletrode wire then it will affect on ph ?
can i use hplc detector to uplc and why?
wen v have polr solvent then which type of column use in hplc
if out of two set of results of moisture analysis first 1 is passing, second one is failing, is it oos ? or vice a versa first 1 is failing and second one is passing. for information mean value to be reported and in mean value the substance is ppassing, is it oos or normaal mean value to be considered?
How is the Flame test performed?
what is the working priniciple of ph, what are parts in ph meter