Answer / nabil

there are many resons for different RT
1-The pressure used(because that affects the flow rate of
the solvent)
2- The nature of the stationary phase(not only what
material is made of, but also particle size.
3-the exact compositionsof the solvent.
4-the temp of the column.

Answer / srinivas

many reaons for different RT in same column
1) Depend on PH in buffer Preparation
2) copmposition of buffer and solvent
3) difference in manual injector and auto injector

Answer / prabhakar

Because preparation of mobile phase variation(mostly)

Answer / dhaval bhatt

1)column temp
2)presser not stable
3)leakage column from any side

Answer / vaibhavgoswami

due to pressure ,
different column packing,(silica)
different maker

Answer / ravi kumar tummala

if mobile not sonicated properly the retention time will vary

Answer / phanindra

I think it is due to contamination of HPLC column remedy is column kept for wash with 100%polar solvent.

HI,I CLEARED BOB CLERK EXAM. MY INTERVIEW WILL BE ON 9TH OCTOBER,2010.PLEASE SEND ME INTERVIEW QUESTIONS AND ANSWERS. THANK YOU.

0 Answers   Bank of Baroda,

In Force degradation Study Compound is not degrade in any condition , also in higher stress what can i do ?

4 Answers   Alembic,

give me method of calculating co relation of coefficient for HPLC,SOR & ANY OTHER

1 Answers  

What is void volume & dead volueme

3 Answers   Bayer, Lupin,

in api coa contains only process impurities and in product coa contains degradation impurities?

0 Answers  

What are the different zones in stability according to ICH?

1 Answers   Apotex,

identification is for unknown? qualification for known? reporting for LOQ?

0 Answers  

what type of question will ask in the interview of lab chemist.

0 Answers   Godavari Biorefineries, Monginis,

what is the definition of limit of stray light in UV calibration

1 Answers   FDC, Zydus Cadila,

verification of wave number why you are use in IR calibration

2 Answers  

How precise should be the linearity in HPLC method validation?

3 Answers   Glenmark,

what is diffrence between specificity and selecivity?

0 Answers